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Assay Protocol for Semiautomated ZIKV Titer Assay

Journal: Assay and Drug Development Technologies

Article Title: High-Throughput Zika Viral Titer Assay for Rapid Screening of Antiviral Drugs

doi: 10.1089/adt.2018.881

Figure Lengend Snippet: Assay Protocol for Semiautomated ZIKV Titer Assay

Article Snippet: ATCC American Tissue Culture Collection DAB diaminobenzidine DMEM Dulbecco's modified Eagle's medium FBS fetal bovine serum FFA focus-forming assay HRP horse radish peroxidase IgG immunoglobulin G LAF laser autofocus MOI multiplicity of infection PBS phosphate buffered saline PBTG phosphate buffered saline with Triton X-100, normal goat serum, and bovine serum albumin PSA penicillin–streptomycin-amphotericin B ZIKV Zika virus

Techniques: Sterility, Incubation, Virus, Concentration Assay, Titration, Immunocytochemistry, Blocking Assay, Staining, Plasmid Preparation